Dengue IgM Capture ELISA

E-DEN01M

Please note these products are not for sale or distribution in the United States and may not be available in all countries worldwide. Please contact Inverness Medical or your local Panbio distributor to determine availability.

Product Overview

The Panbio dengue IgM Capture ELISA is for the qualitative detection of IgM antibodies to dengue antigen in serum, as an aid in the clinical laboratory diagnosis of patients with clinical symptoms consistent with dengue fever.

Detection of IgM antibodies to dengue virus by ELISA is a valuable procedure, particularly in second and subsequent infections where the occurrence of complications is high. Serum IgM antibodies can be detected from dengue patients as early as three to five days after the onset of fever and generally persist for 30 - 90 days, although detectable levels may be present eight months post-infection.

The IgM cut-off is set to detect IgM levels characteristic of current dengue infection.

  • As an aid in the diagnosis of acute dengue
  • Suitable for use in endemic and non-endemic dengue regions
  • Recommended for use with the Dengue IgG Capture ELISA in regions where secondary dengue is prevalent

Quick Info

  • Excellent performance
  • Standardised method
  • Total incubation time 2 hr 10 min
  • Break-apart wells
  • Ready-to-use colour coded reagents
  • Compatible with standard microplate technology
  • 96 tests

Performance

Dengue IgM Capture and Dengue IgG Capture ELISA serological sensitivity and specificity1
Serological sensitivity IgM 95% CI* IgG 95% CI*
Primary dengue
54/57 (94.7%)
85.4-98.91%
 N/A
N/A
Secondary dengue2 64/115 (55.7%)3 46.6-64.7% 52/54 (96.3%) 73.8-93.6%
Serological specificity IgM 95% CI* IgG 95% CI
Negatives
83/83 (100%)
95.7 - 100 % 125/125 (100%) 97.1-100%
Primary dengue N/A 95.7 - 100 % 32/35 (91.4%) 76.9-98.2%
  1. This data has been extracted from the Dengue IgM Capture and Dengue IgG Capture ELISA Instructions for Use.
  2. Samples positive for secondary dengue infection by HAI (titre >= 2560)
  3. The use of the Panbio Dengue IgG Capture ELISA (E-DEN02G) is recommended when secondary dengue is suspected

* Confidence Interval
Cross-reactivity data - Panbio Dengue IgM Capture ELISA1
Disease type Total specimens Positive (or equivocal) result
Epstein-Barr virus 10 0/10
Malaria 10 1/10
Influenza A 7 0/7
Influenza B 3 0/3
Anti-nuclear antibody 30 0/30
Rheumatoid factor 10 3/10
Hepatitis A 9 0/9
Leptospira 7 0/7
Salmonella typhi 9 0/9
Scrub typhus 10 0/10
West Nile virus 10 2/10
TOTAL 115 6/115

Publications

Blacksell, S. et al (2007) Evaluation of the Panbio dengue virus nonstructural 1 antigen detection
and immunoglobulin M antibody enzyme-linked immunosorbent assays for the diagnosis of acute dengue infections in Laos. Diagnostic Microbiology and Infectious Disease 60:43-49

Cuzzubbo, A., et al. (1997). Commercial Assays for the Serological Diagnosis of Dengue Infection. Arbovirus Research in Australia. 7:56-60. Queensland Institute of Medical Research.

Cuzzubbo, A., et al. (1999) Comparison of PanBio Dengue Duo Enzyme-Linked Immunosorbent
Assay (ELISA) and MRL Dengue Fever Virus Immunoglobulin M Capture ELISA for Diagnosis of Dengue Virus Infections in Southeast Asia. Clinical and Diagnostic Laboratory Immunology Sep. 1999:705-712

Groen, J., et al (2000) Evaluation of Six Immunoassays for Detection of Dengue Virus-Specific Immunoglobulin M and G Antibodies. CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY,Nov. 2000, p. 867–871

Lam, K., et al (2000) Evaluation of a Capture Screening Enzyme-Linked Immunosorbent Assay for Combined Determination of Immunoglobulin M and G Antibodies Produced during Dengue Infection. Clinical and Diagnostic Laboratory Immunology Sep. 2000:850-852

Phillips, D., et al (1997) Flavivirus Diagnostic Assays: The Queensland Perspective. Arbovirus Research in Australia. 7:208-211h. Queensland Institute of Medical Research.

Sang, C., et al (1998) Evaluation of a Commercial Capture Enzyme-Linked Immunosorbent Assay for Detection of Immunoglobulin M and G Antibodies Produced during Dengue Infection. Clinical and Diagnostic Laboratory Immunology Jan. 1998: 7-10

Vaughn, D., et al (1999) RAPID SEROLOGIC DIAGNOSIS OF DENGUE VIRUS INFECTION USING A COMMERCIAL CAPTURE ELISA THAT DISTINGUISHES PRIMARY AND SECONDARY INFECTIONS. Am. J. Trop. Med. Hyg. 60(4): 693-698

Vazquez, S., et al (2007) Evaluation of immunoglobulin M and G capture enzyme-linked
immunosorbent assay Panbio kits for diagnostic dengue infections. Journal of Clinical Virology 39:194-198

Instructions for use

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